The role of time pressure steam sterilization
Autoclave made basic elements include the three elements of reaction time, temperature and saturated steam sterilization and the like.
Microbial heat tolerance varies depending on the type, therefore, according to the degree of contamination sterilization items, the required sterilization temperature and time vary, and the selection of sterilization temperature and time required with sterilization, items performance, packaging materials, the requirements on the length of the sterilization process. Generally the higher the sterilization temperature, the shorter the time required for saturated steam temperature and pressure was constant relationship, but the cabinet did not rule out the indoor air or not fully ruled out, the steam can not reach saturation, although this time the pressure The table below shows the sterilization pressure has reached, but did not meet the requirements of the steam temperature, resulting in sterilization failure.
Saturated steam must meet dried (moisture-containing <5%> and clean (including cold <3.5%>, superheat should not exceed 5 ℃, because in case of saturated steam is condensed to release large amounts of latent heat, so the temperature rises items ; condensed at the same time, the volume contracted sharply (1/1600), but also generate local negative pressure, so that the subsequent steam penetrates into the depths of the articles of superheated steam, condensate slowdown on the one hand, is not conducive to sterilization, this phenomenon overheating That performance was more than 5 ℃. in addition, the steam superheater can lead to rapid aging items. pressure superheated steam generation system or gas may be due to decreased provisions, or elevated temperature or textile jacket is too dry. when the steam contains more subtle fog, etc., form a barrier to gas flow without heat penetration, but also increase the difficulty after sterilization drying process. the main result of the presence of fog particles in excess boiler water surface blisters is quickly burst out of steam, or steam condensate through a long pipeline from when the relationship between saturated steam steam mixed with air pressure and temperature between the time deviates, then the pressure gauge should be deducted from the value shown in the air partial pressure, that it can reach sterilization temperature will decrease, such as not pay attention to this, because the result did not achieve sterilization temperature sterilization failure. Autoclaves steam mixed with air may be due to gas system, the original cabinet room air or air inside the sterilized items produced is not excluded, the presence of air, but also because of the air by steam extrusion forming an air column of air in contact barrier steam sterilization items, thus affecting the steam sterilization items internal penetration, the formation of low-temperature package, outside of the package at a higher temperature unevenness of the situation, so that the internal temperature does not reach the sterilization; and because of the presence of air, reducing the water content of the gas sterilization chamber, namely the level of humidity reduced, is not conducive to kill the micro-killing substance.
The role of time pressure steam sterilization, should sterilizer large indoor temperature to the required sterilization date until sterilization is complete. Total time includes: heat penetration time, which reached sterilization temperature sterilization cabinet to sterilized items from the central portion of the sterilization temperature has reached the required time, the length of time depends on the nature of the sterilized items, the package size, placement position, the air inside the sterilization degree of evacuation; sterilization duration, i.e., to kill microorganisms require extended heating time, generally half of the sustain time, depending on the length of the article may be sterilized; and the time required for sterilization treatment : warm-up time, the cold air discharge time, the cabinet room heating time, cooling time required for the sterilization process ends. Warm-up time refers to the indoor heated sterilizer sterilization temperature reaches a predetermined time, preheat uniformity increase the indoor cabinet dry heat and steam to prevent excessive condensation; exclude cold time of the original cabinet interior cool air discharge outside the cabinet, two biological safety cabinets to enhance the penetration of heat, which is particularly important for steam, should be given sufficient time; cabinet room heating time means items will put the entire cabinet is heated to room temperature sterilization requires time; cooling time means after the completion of disinfection or sterilization heating is stopped, the temperature dropped to the cabinet room safe time to open the door.
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